Part:BBa_K4737010

pnirB::GFP

To express GFP in the anaerobic environment

Usage and Biology

While anti-CEA scFv can enhance the targeting ability of VNP20009 towards tumor cells, we aim to mitigate the toxic effects of this bacterium on normal tissue. To further enhance the safety of our project, we need to screen specific promoters that activate exclusively within the tumor microenvironment (TME). In solid tumors, the tumor tissue grows rapidly, leading to significant inflammation, and the vascular system within the tissue is incomplete. Consequently, the tumor microenvironment often exhibits characteristics indicative of overall oxygen deficiency. The nirB promoter is the promoter of the first gene of E. coli NADH-dependent nitrite reductase operon. This promoter is induced in anaerobic conditions and up regulated in response to nitrite and nitrate. Its synthesis mechanism has been thoroughly studied and has been chosen by numerous iGEM teams (e.g., Nanjing_China_Bio 2012, TecMonterrey 2013, etc.) to mitigate the toxic effects of bacteria on normal tissue. Based on the aforementioned considerations, we have selected the nirB promoter to initiate subsequent killing modules.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1051